- Systems and Solutions for Bioscience Research
MicroBrightField Stereology Discussion
Physical Disector Counting Query
Friday 13 July 2007, @11:38AM by Fahd Jowhari
Glomerular Counting suggestion reqd!
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Blue dots and jerky movement in the live image
Tuesday 10 July 2007, @02:14AM by Jessica Barnes
Stereo Investigator has blue dots on the live image and the live image does not move smoothly when navigating around a section.
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Frozen vs. plastic sections with optical fractionator?
Sunday 19 February 2006, @03:28PM by Lyngine Calizo
Will using frozen sections instead of plastic sections result in more variation in the estimate of neuron number? The sections will be Nissl stained. The probe to be used is the optical fractionator.
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To include an optical disector that lands on the edge?
Tuesday 18 October 2005, @09:44PM by Steven S
What are the rules for including a disector in counts if it falls on the edge of your reference space?
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(2 comments)
Shortcut for selecting Marker?
Thursday 06 October 2005, @04:44AM by christian
Looking for a quick way to select markers with a keyboard shortcut
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(1 comment)
Calculating total counting frame area
Thursday 25 August 2005, @02:39PM by Eric
Correctly estimating total area of counting frames examined
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(1 comment)
I can't see my image
Wednesday 13 July 2005, @09:49AM by hong meng
when I turn on everything and I can't see the image on the screen.
I don't know why.
It would be great apprciated if somebody tell me how to do it.
Thanks.
Hong
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(1 comment)
simple SRS 2D count, testfields, counting frame, CE
Thursday 30 June 2005, @08:12AM by Chris
Being unexpierenced with the Fractionator, having questions about simple SRS 2D count, testfields, counting frame & CE
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(1 comment)
Total counts
Thursday 30 June 2005, @03:58AM by Soumya
Total neuron counts in substantia nigra, pars compacta of mice
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(1 comment)
Neuronal counting
Tuesday 21 June 2005, @10:12PM by Zunji Ke
Hi, All,
I am planning to have a set of stereological equipment for counting neurons in the brain in my new lab in China. Any suggestion are more than welcome. Thank you so much. Have a nice day!
Zunji
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Large Particles
Tuesday 26 April 2005, @03:16PM by Stephen Kanes
I am interested in unbiased counting of olfactory glomeruli in mouse olfactory bulb.
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(1 comment)
circular contours
Wednesday 20 April 2005, @02:02PM by Avinash Budhian
To be able to delineate a region of interest such that it is a circle of specified radius from a specified point, instead of drawing coutours by hand.
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population count vs population estimate not working
Friday 14 January 2005, @05:42PM by Zack Kielich
Sample populations not showing same results as actual population.
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(4 comments)
does size matter?
Wednesday 15 December 2004, @10:27AM by Emma Lane
How can i accurately count and compare striatal grafts of different sizes?
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(1 comment)
Variable number of sections ok?
Thursday 28 October 2004, @05:25PM by denise @ UCLA
Is it ok to count a different number of sections for each animal within a group?
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(2 comments)
SI Reference point issues
Wednesday 20 October 2004, @10:31AM by Sharon Reimsnider
reference point resets...
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Optimal amount of sampling sites
Tuesday 12 October 2004, @06:04PM by Zack Kielich
I'm in the process of writing a protocol for my lab on the use of StereoInvestigator's optical fractionator function and can't find any info on the optimal amount of sampling sites.
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(1 comment)
tissue thickness
Monday 11 October 2004, @07:19PM by Rechal
Tissue thickness a limitation
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(1 comment)
CE
Monday 04 October 2004, @07:33PM by Ngan Doan
Which CE do I use?
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(1 comment)
Cruz-Orive versus Gundersen's CE
Monday 27 September 2004, @03:17AM by Oliver von Bohlen
What is the reason using the CE of Cruz-Orive instead of the Gundesen's CE?
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(1 comment)
Nested contours
Tuesday 21 September 2004, @06:30PM by Denise @ UCLA
Need to exclude a contour within a contour
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(4 comments)
What is scan length?
Monday 26 July 2004, @03:49PM by Eric
Under display probe run list to obtain final results...and then under linear scan...there is a result that is titled "scan length"...what is this value?
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Exporting NeuroExplorer "rotation motor tool" images
Thursday 08 July 2004, @12:21AM by Tracey
How can I export the rotating image of my traced neuron into another program?
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Delimiting nucleus accumbens
Tuesday 06 July 2004, @07:56AM by A. Franky
Dificulties on the delimitation of nucleus accumbens
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(2 comments)
Calculation of Neuron Density
Friday 23 April 2004, @07:48AM by Katherine Day-Wilson
Calculation of neuron density by dividing estimated total neuron number by the estimated volume.
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(1 comment)
Jugular Ganglia Neuron Counts
Thursday 15 April 2004, @11:47AM by Dhyan Rajan, West Virginia University
Neuron counts in Jugular Ganglia Using O.F.
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Different Types of CE
Monday 05 April 2004, @12:51AM by sarah kay
What are the differences between Gundersen, Scheaffer and Schmitz-Hof CE estimates?
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Cell counts in a cannulated hippocampus
Thursday 04 March 2004, @10:53AM by Dan McCloskey
When a cannula is placed into the hippocampus, do the same rules apply to calculating neuron number?
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(1 comment)
Neuronal Count in Ganglia
Tuesday 03 February 2004, @10:29AM by Dr. Dhyan Rajan
CE of OF
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(2 comments)
CE or CV
Tuesday 20 January 2004, @02:46AM by Ana Jongen
Ce or CV: what to be??
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(1 comment)
HELP
Tuesday 13 January 2004, @12:23PM by Misty Ryan
I have no clue how to use the optical fractionator. Can somebody please run through some step by step directions for me?
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(2 comments)
Stereology for counting dendrites?
Monday 17 November 2003, @06:09PM by David Lyons
Can stereology by used to score hippocampal atrophy within a given multi-neuron region, e.g., CA3?
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(2 comments)
Use of optical fractionator in IF stained frozen section on fluorescence microscope ?
Wednesday 22 October 2003, @06:45AM by liesbeth forsthuber
what are the requirements to use the optical fractionator on immunoflourescence stained neurons (neuronal nuclei) in frozen sections on a flourescence microscope ? What could be the major problems arising ? can anyone recommend published papers using these conditions (in any brain region) ?
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(1 comment)